23052 - Bismuth Sulfite Agar (BS) - Môi trường thạch Bismuth Sulfite Agar (BS)

023052 Bismuth Sulfite Agar (BS)
Usages: For selective isolation of Salmonella spp, especially for Salmonella typhi.
Principle:
Peptone, beef extract powder provides carbon, nitrogen, vitamins and minerals; glucose to provide energy; bismuth sulfite indicator suppress gram-positive bacteria and coliform bacteria, but does not affect the growth of salmonella; agar as culture medium coagulant.
Formulation（per liter）:
Peptone 10g
Beef extract powder 5g
Dextrose 5g
Ferrous sulfate 0.3g
Disodium hydrogen phosphate 4g
Sodium sulfite  6g
Bismuth ammonium citrate  2g
Brilliant green  0.025g
Agar 15g
Final pH7.2 ± 0.2
How to use：
1.Suspend 47.3g in 1L of distilled water ,stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
 Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
 Specifications: 250g/bottle