HCM015 - Môi Trường Urea Agar Base - Môi Trường christensen

HCM015 Urea Agar Base

Usages:For differentiating enteric bacteria based on urease activity by adding 40% sterile urea solution.
Principle:
Peptone provides the carbon and nitrogen; maintain a balanced osmotic sodium chloride; potassium dihydrogen phosphate is buffers; decomposing bacteria urease urea medium, produce large amounts of ammonia, agar as medium coagulant.
 Formulation (per liter):
Peptone 1g
Sodium chloride 5g
Glucose 1g
Ppotassium dihydrogen phosphate 2g
Phenol red 0.012g
Agar 12g
Final pH7.2 ± 0.2
 How to use:
1.Suspend 21g in 1L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes. cooling to 50-55 ℃ and adding 40% urea solution.
2.Diluted and treated samples.
 Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
 Specifications: 500g/bottle
*Supplement : 029100 40% urea solution